Journal: Neoplasia (New York, N.Y.)

Article Title: Multi-omics profiling reveals microenvironmental remodeling as a key driver of house dust mite-induced lung cancer progression

doi: 10.1016/j.neo.2026.101275

Figure Lengend Snippet: HDM d oes not have mutagenic effects in human and mouse lung epithelial cell lines i n v itro . A) Dose-response curves of HDM treatment in BEAS-2B and LLC cells. Cell viability is shown relative to vehicle control (PBS; % of control). Calculated IC 50 values are indicated. B) Experimental setup for HDM exposure and duplex sequencing. BEAS-2B and LLC cells were treated with either HDM (200 µg/mL for BEAS-2B; 400 µg/mL for LLC) or vehicle (VEH; PBS), expanded across multiple passages, and subsequently subjected to genomic sequencing. C) Comparison of mutational burden between samples treated with either HDM (purple) or VEH (blue), shown as somatic mutations per megabase (Mb) of coding DNA. D) Mutational spectra of BEAS-2B and LLC cells treated with HDM compared to VEH controls. Mutations are classified into the six base substitution categories and displayed in SBS96 format. The experiments were performed in triplicate (A) or singlicate (B-D).

Article Snippet: Human: BEAS-2B cells , ATCC , Cat# CRL-3588, RRID:CVCL_0168.

Techniques: Control, Sequencing, Genomic Sequencing, Comparison

Journal: Biochemistry and Biophysics Reports

Article Title: Preliminary study of cyto-impedance: Molecular profiling of functional impedance in motility-promoting treatment of normal cells

doi: 10.1016/j.bbrep.2026.102476

Figure Lengend Snippet: EH-P002A promotes wound healing in a dose-dependent manner . A. Molecular structure of EH-P002A. B. No detectable cytotoxicity was observed in HFF-1 or Beas-2B cells upon EH-P002A treatment. C. Wound images from mice treated with EH-P002A at two doses, captured at different time points. D. Wound healing rates on Days 7 and 9. Dose-dependent efficacy on Day 7 was observed.

Article Snippet: Human fibroblast cell line HFF-1 (Cat. No. SCRC-1041, ATCC) and bronchial epithelial cell line Beas-2B (Cat. No. CRL-3588, ATCC) were cultured in Dulbecco's Modified Eagle Medium supplemented with 10 % fetal bovine serum in a humidified 5 % CO2 incubator at 37 °C, and confirmed to be free of mycoplasma contamination.

Techniques:

Journal: Biochemistry and Biophysics Reports

Article Title: Preliminary study of cyto-impedance: Molecular profiling of functional impedance in motility-promoting treatment of normal cells

doi: 10.1016/j.bbrep.2026.102476

Figure Lengend Snippet: Dose-dependent cyto-impedance induced by EH-P002A . A . Wound-healing assay images of HFF-1 cells treated with indicated concentrations of EH-P002A, followed by quantification in the right panel. A promoting effect on wound healing was observed at 8 μM, while overdosage of EH-P002A suppressed the migratory ability. B . Images of migrated HFF-1 cells in transwell assays treated with varying EH-P002A concentrations followed by quantification in the right panel, showing promotion at 8 μM and suppression at higher doses. C. The data of wound-healing assay of Beas-2B cells were quantified, showing a promoting effect at 8–20 μM concentrations, and a suppressive effect at a higher concentration of 80 μM. D . Quantification of the transwell assays of Beas-2B cells confirmed dose-dependent cyto-impedance at 40 and 80 μM concentrations. ∗, P < 0.05; ∗∗, P = 0.001; ∗∗∗, P < 0.001.

Article Snippet: Human fibroblast cell line HFF-1 (Cat. No. SCRC-1041, ATCC) and bronchial epithelial cell line Beas-2B (Cat. No. CRL-3588, ATCC) were cultured in Dulbecco's Modified Eagle Medium supplemented with 10 % fetal bovine serum in a humidified 5 % CO2 incubator at 37 °C, and confirmed to be free of mycoplasma contamination.

Techniques: Wound Healing Assay, Concentration Assay